Cell Culture Vessel
Standard Tissue Chambers
Our standard tissue chambers are uniquely flexible in their use. We’ve incorporated standard laboratory consumbles in order to improve the transition of manual protocols into a fully automated solution in an OSPIN bioreactor.
Laminar Flow Chamber
The shear flow chamber has been designed to expose adherent cells to flow induced shear stress. Cells can be cultivated on a simple polystyrene sheet before being placed in the culture chamber. Like usual well-plates or microscope slides, it can be functionalized and used according to existing protocols. The cell culture medium flowing through the chamber induces a shear force on the cells in culture.
Multi-well Plate Automation
Using standard multi-well plates you automate the fresh supply of medium to each of the wells while relying on your established protocols for seeding, treatment and harvest of cells, gels and scaffolds. The system is designed to be compatible with 6-well-plates. The plates bottom part is still accessible for visual control using an inverted microscope. All medium perfusion and gas exchange is performed through a custom-made lid to the well-plate.
Cell Insert Chamber
Cell insert systems are an established method to study in vitro drug transport, migration and cell invasion. Our tissue chamber acts as a casing for cell inserts, e.g. Corning transwell system. This means you can easily apply already established protocols while taking advantage of automatic exchange of cell culture medium on the basolateral side. Using perfusion in this way allows for slow, but constant, medium exchange as well as application of shear stress on cells in the basolateral chamber.
Custom-made tissue chambers
Our team has several years of experience in designing tissue chambers for very specific purposes.Chambers can for example incorporate electrical and mechanical stimulation as well as different wells to perform measurements in parallel. Here are some examples:
Dual Side Perfusion
For one of our customers, we developed a system to allow for dual side perfusion of a hydrogel. In this case we developed a chamber allowing for dual side perfusion of a hydrogel, where osteocytes, chondrocytes and mesenchymal stem cells can be co-cultivated. By encasing the scaffold between two membranes and adding perfusion on the outwards facing sides of the membrane, we are able to supply nutrients to the cells in a way that recreates the physiological conditions of cartilage.
Cardiomyocyte stimulation & force measurement
We also have developed a tissue chamber that allows for the mechanical stimulation of cardiomyocytes and the measurement of the force they are create. The cells can be periodically stretched while the contractile force of the cells attached can be measured.
Contact us to learn more!
Our team the here for you to discuss which is the best vessel for your experiments – standard or custom-made!